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Research Technician - Knoechel

Job Description

Research Technician - Knoechel

Job ID:



450 Brookline Ave, Boston, MA 02215


Research Laboratory

Employment Type:

Full time

Work Location:

Onsite:Up to 1 day remote/wk


The Dana-Farber Cancer Institute is looking for an exceptional candidate for a unique Research Assistant position. This position affords the exciting opportunity to be part of a research effort at Dana-Farber Cancer Institute aiming to delineate epigenetic heterogeneity in cancer and its influence on the leukemia microenvironment and development of novel immunotherapies. The candidate will assist in the development of cancer models from primary human leukemias and human cell lines that represent different cellular lineages and drug-resistant states. The research technician will use modern next generation sequencing techniques to characterize the transcriptional and epigenetic state of these leukemias and modern functional perturbation techniques including lentiviral knockdown and CRISPR/Cas9 genome editing tools. He/she might work with disease-relevant mouse models. This role will be part of a multi-disciplinary team that evaluates new technologies and approaches to discover and validate novel systemic cancer biology.

The candidate is expected to work largely independently after training but will be closely mentored by the principal investigator and senior postdoctoral research fellows in the lab.

Dana-Farber Cancer Institute provides a vibrant research environment with close links to top academic institutions across the Boston and Cambridge area and provide the potential for your contributions to be used and recognized worldwide.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute is a leader in life changing breakthroughs in cancer research and patient care. We are united in our mission of conquering cancer, HIV/AIDS and related diseases. We strive to create an inclusive, diverse, and equitable environment where we provide compassionate and comprehensive care to patients of all backgrounds, and design programs to promote public health particularly among high-risk and underserved populations. We conduct groundbreaking research that advances treatment, we educate tomorrow's physician/researchers, and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.


  • Learn, execute and further develop next-generation sequencing protocols and explore novel techniques of low input epigenomics and single cell sequencing.
  • Handles cell culture of human cancer cell lines and primary human cells. Uses established protocols for lentiviral knockdown, CRISPR/Cas9 genome editing, overexpression.
  • Uses wide assay of cell biology and molecular biology techniques to mechanistically characterize dependencies including multi-dimensional flow cytometry.
  • Learn and execute a variety of in vitro functional bioassays and high dimensional phenotypic readouts.
  • May work with mouse models to assess tumor formation, and drug response.
  • Operates and maintains laboratory equipment to complete investigations.
  • Prepares, orders, and maintains stocks of necessary reagents, solutions and supplies.
  • Documents, compiles, and analyzes experimental protocols and data including running computational analysis pipelines on the command line.
  • Attends team meetings to share results, plan projects and experiments.
  • Other related tasks as required.


  • Bachelor degree in Biology, Computational Biology or related field required. 0-2 yrs of experience working in a scientific research setting a plus.
  • Familiarity of basic cell culture and molecular biology laboratory techniques, including recombinant DNA and protein biochemistry, strongly desired.
  • Familiarity with fluorescent microscopy or flow cytometry is desirable.
  • Familiarity with the command line, linux and R desirable.
  • Strong interpersonal, communication, and organizational skills, including the ability to handle a variety of tasks in a fast-paced environment, large complex experiments, and large data sets, all of which demand great attention to detail.
  • Proactive in identifying problems and solutions to address them.
  • Must be an exceptional team player.
  • Must possess a high level of initiative and the ability to work independently.

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.

At Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusive environment where every patient, family, and staff member feels they belong. As relentless as we are in our mission to reduce the burden of cancer for all, we are equally committed to diversifying our faculty and staff. Cancer knows no boundaries and when it comes to hiring the most dedicated and diverse professionals, neither do we. If working in this kind of organization inspires you, we encourage you to apply.


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